Abstract
Lactarius pseudoscrobiculatus is a rare Mediterranean species defined on the basis of morphological characters, and reported from coastal areas of south France, northeast Spain and northwest Italy growing in association with maritime pines (Pinus pinaster and P. halepensis). Prior to the present study, no data existed about its phylogenetic position and the relationships to other taxa of the genus. In the frame of this work, the morphoanatomical features of two pertinent collections from Ikaria Island (East Aegean, Greece) and the area of Lucca (Tuscany, Italy) were studied in detail and compared to the original description. The respective material as well as the type specimen were also subjected to ITS rDNA sequencing. Phylogenetic analysis demonstrated that all examined specimens nested within a well-supported clade corresponding to L. pseudoscrobiculatus which is positioned among other species of Lactarius subsect. Scrobiculati. In addition, L. pseudoscrobiculatus formed a sister group with L. citriolens, evidencing their relative affinity. The confirmed presence of L. pseudoscrobiculatus in the East Mediterranean in association with P. brutia and Cistus spp. expands existing knowledge on the geographic distribution and ecology of this ectomycorrhizal species.
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Acknowledgements
E.P. was financially supported through an IKY scholarship (MIS 5001552) co-financed by E.U. (European Social Fund—ESF) and Greek national funds through the action ‘Reinforcement of Postdoctoral Researchers’ of the Operational Programme ‘Human Resources Development Program, Education and Lifelong Learning’ of the National Strategic Reference Framework (NSRF) 2014–2020. We would like to thank the reviewers for their constructive comments.
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Polemis, E., Nuytinck, J., Fryssouli, V. et al. Phylogeny, ecology and distribution of the rare Mediterranean species Lactarius pseudoscrobiculatus (Basidiomycota, Russulales). Plant Syst Evol 305, 755–764 (2019). https://doi.org/10.1007/s00606-019-01604-3
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DOI: https://doi.org/10.1007/s00606-019-01604-3